Shih-Tong Jeng Professor
(College of Life Science Dean)
Ph.D., Department of Biochemistry, University of Illinois at Champaign-Urbana
Specialty: Plant Stress Physiology, Plant Molecular Biology, Small
Laboratory: Life Science Building R1018
Telephone: 886-2-3366 2538
Current Research Interests
Function of hydrogen peroxide-regulated small RNAs in rice.
Isolation and characterization of small RNAs and genes regulatedby systemic wounding in sweet potato.
Gene regulation and signal transduction in plants.
zong yan liu
Function of hydrogen peroxide-regulated small RNAs in rice. Rice, a major grain crops in the world, is grown in the field, and is always exposed to various environmental stresses. The stress environment induces plant to produce reactive oxygen species (ROS), including H2O2 . The appropriate concentration of H2O2 acts as a signal messenger, while the excessive ROS causes the oxidative stress and cell death. Hence, this research is to study the functions of H2 O2-related small RNAs and their target genes in rice. Small RNA sequencing and degradome sequencing were already used to screen H2O2-related small RNAs and their target genes. Further through the information of microarray from rice treated with H2O2, several miRNA/target modules were selected. The quantitative RT-PCR and agroinfiltration were used to confirm the relationship between small RNAs and their targets. This study may elucidate the functions of H2O2-related miRNAs and their target genes in rice, and can be further applied to increase the tolerance of rice under environmental stresses.
Isolation and characterization of small RNAs and genes regulated by systemic wounding in sweet potato. Plants have developed defense systems responding to external attacks in order to survive from herbivores. After wounding, the leaves physically wounded are called local leaves, and the leaves, which are not wounded but in the same plant, are named systemic leaves. The mechanism regulated by the systemic leaves in sweet potato after wounding is under studied. The expression of peptide hormone gene preproHypSys is induced in sweet potato after wounding and its processing product Hydroxyproline-rich glycopeptides (HypSys) is able to stimulate the systemic effect over all the plant. Transgenic sweet potato overexpressing preproHypSys has been constructed, and is able to provoke very strong systemic signal after wounding. Therefore, this transgenic sweet potato is an excellent material to investigate systemic wounding effect. The results of this study may uncover the regulation network of systemic wounding, and may facilitate plants to survival from herbivore attack.
1. Tseng, B.-S., Huang, C.-C., King , Y.-C., Wu, M.-T., Hsieh, C.-H., Hsieh, K,-T., Hsing, Y.-I., Jeng., S,-T,* (2023) Hydrogen peroxide regulates the Osa-miR156-OsSPL2/OsTIFY11B module in rice. Plant, Cell and Environment 46, 2507-2522.
2. Tsai, W.-A., Sung, P.-H., Kuo, Y.-W., Chen, M.-C., Jeng, S.-T.*, Lin, J.-S.* (2023). Involvement of microRNA164 in responses to heat stress in Arabidopsis. Plant Science, 329: 111598.
3. Weng, S.-T., Kuo, Y.-W., King, Y.-C., Lin, H.-H., Tu, P.-Y., Tung, K.-S., Jeng, S.-T. * (2020) Regulation of microRNA2111 and its target IbFBK in sweet potato on wounding. Plant Science 292, 110391.
4. Lin, H.-H., King, Y.-C., Li, Y,-C., Lin, C.-C., Chen, Y,-C., Lin, J.-S. *, Jeng, S.-T. * (2019) The p38-like MAP kinase modulated H2O2 accumulation in wounding signaling pathways of sweet potato. Plant Science 280, 305-313.
5. Kuo, Y.-W., Lin, J.-S., Li, Y.-C., Jhu, M.-Y., King, Y.-C., Jeng, S.-T. * (2019) MicroR408 regulates defense response upon wounding in sweet potato. Journal of Experimental Botany 70, 469-483.
6. Lin, J.-S., Kuo, C.-C., Yang, I.-C., Tsai, W.-A., Shen, Y.-H., Lin, C.-C., Liang, Y.-C., Li, Y.-C., Kuo, Y.-W., King, Y.-C., Lai, H.-M., Jeng, S.-T. * (2018) MicroRNA160 modulates plant development and heat shock protein gene expression to mediate heat tolerance in Arabidopsis, Frontiers in Plant Science 9, 68.
7. Lin, M.-C., Tsai, H.-L., Lim, S.-L., Jeng, S.-T., Wu, S.-H. (2017) Unraveling multifaceted contributions of small regulatory RNAs to photomorphogenic development in Arabidopsis. BMC Genomics, 18, 559.
8. Cheng, N., Lo, Y.-S., Ansari, M. I., Ho, K.-C., Jeng, S.-T., Lin, N.-S., Dai, H. (2017) Correlation between mtDNA complexity and mtDNA replication mode in developing cotyledon mitochondria during mung bean seed germination. New Phytologist, 213, 751-763.
9. Wang, H.-M., Jeng, S.-T., To, K.-Y. (2017) In vitro regeneration, Agrobacterium-mediated transformation, and genetic assay of chalcone synthase in the medicinal plant Echinacea pallida. Plant Cell, Tissue Organ Culture 130, 117-130.
10. Cheng N., Lo, Y.-S., Ansari, M. I., Ho, K.-C., Jeng, S.-T., Lin, N.-S., Dai, H. (2017) Correlation between mtDNA complexity and mtDNA replication mode in developing cotyledon mitochondria during mung bean seed germination. New Phytologist, 213, 751-763.
11. Li, Y.-C., Wan,W.-L., Lin, J.-S., Kuo, Y.-W., King, Y.-C., Chen, Y.-C., Jeng, S.-T.* (2016) Signal transduction and regulation of IbpreproHypSys in sweet potato. Plant Cell and Environment 39, 1576-1587.
12. Wang, H.-M., To, K.-Y., Lai, H.-M., Jeng, S.-T. * (2016) Modification of flower color by suppressing β-ring carotene hydroxylase genes in Oncidium. Plant Biology 18, 220-229.
13. Kuo, T. C.-Y., Chen, C.-H., Chen, S.-H., Lu, I.-H., Huang, L.-C., Lin, C.-Y., Chen, C.-Y., Lo, H.-F.,Jeng, S.-T., Chen, L.-F. O. *. (2015) The Effect of Red Light and Far-Red Light Conditions on Secondary Metabolism in Agarwood. BMC Plant Biology 15, 139.
14. Chen, C.-H., Kuo, T. C.-Y., Yang, M.-H., Chien,T.-Y., Chu, M.-J., Huang, L.-C., Chen, C.-Y., Lo, H.-F., Jeng, S.-T., Chen, L,-F, O. * (2014) Identification of cucurbitacins and assembly of a draft genome for Aquilaria agallocha. BMC Genomics 15, 578.
15. Lin, J.-S., Lin, H.-H., Li, Y.-C., King, Y.-C., Sung,R.-J., Kuo,Y.-W., Lin, C.-C., Shen, Y.-H., Jeng, S.-T.* (2014) Carbon monoxide regulates the expression of a wound-inducible gene Ipomoelin through antioxidation and IbMAPK phosphorylation in sweet potato. Journal of Experimental Botany 65, 5279-5290.
16. Shen, Y.-H., Chen, Y.-H., Liu, H.-Y., Chiang, F.-Y., Wang, Y.-C., Hou, L.-Y., Lin, J.-S., Lin, C.-C., Lin, H.-H., Lai, H.-H., Jeng, S.-T. * (2014) Expression of a gene encoding β-ureidopropionase is critical for pollen germination in tomatoes. Physiologia Plantarum 150, 425-435.
17. Lin, J.-S., Lin, C,-C, Li,Y.-C., Wu, M.-T., Tsai, M.-H. Hsing, Y.-I. C. and Jeng, S.-T.* (2013) Interaction of small RNA–8105 and the intron of IbMYB1 RNA regulates IbMYB1 family genes through secondary siRNAs and DNA methylation after wounding. The Plant Journal 75, 781-794.
18. Lin, J.-S., Lin, C.-C., Lin,H.-H., Chen, Y.-C. and Jeng, S.-T.* (2012) MicroR828 regulates lignin and H2O2 accumulation in sweet potato on wounding. New Phytologist 196, 427-440.
19. Lee, C.-C., Ho, H., Jeng, S.-T., Chiang, B.-L. (2011) Construction of Der p2-transgenic plant for the alleviation of airway inflammation. Cellular & Molecular Immunology 8, 404-414.
20. Lin, C.-C., Chu, C.-F., Liu, P.-H., Lin, H.-H., Liang, S.-C., Hsu, W.-E., Lin, J.-S., Wang, H.-M., Chang, L.-L., Chien, C.-T., Jeng, S.-T.* (2011) Expression of an Oncidium Gene Encoding a Patatin-Like Protein Delays Flowering in Arabidopsis by Reducing Gibberellin Synthesis. Plant and Cell Physiology 52, 421-435.
21. Lin, C.-C., Jih, P-J, Lin, H.-H., Lin, J.-S., Chang, L.-L., Shen, Y.-H., Jeng, S.-T. * (2011) Nitricoxide activates superoxide dismutase and ascorbate peroxidase to repress the cell death induced by wounding. Plant Molecular Biology 77, 235-249.
22. Lin, H.-H., Huang, L.-F., Su, H.-C. and Jeng,S.-T.* (2009) Effects of the multiple polyadenylation signal AAUAAA on mRNA 30-end formation and gene expression. Planta 230, 699-712.
23. Chen, Y.-C., Lin, H.-H. and Jeng, S.-T.* (2008) Calcium influxes and mitogen-activated protein kinase kinase activation mediate ethylene inducing ipomoelin gene expression in sweet potato. Plant, Cell and Environments 31, 62-72.
24. Chen, H.-F. Chang, M.–H., Chiang, B.-L*. Jeng, S.-T.* (2006) Oral immunization of mice using transgenic tomato fruit expressing VP1 protein from enterovirus. Vaccine 24, 2944-2951.
25. Liu, Y.-C., Lin, S.-H., Lai, H.-M., and Jeng, S.-T.* (2005) Detection of Genetically modified soybean and its product tou-kan by polymerase chain reaction with dual pairs of DNA primers. European Food Research and Technology 221, 725-730.
26. Chen ,Y.-C., Chang, H.-S., Lai, H.-M. and Jeng,S.-T.* (2005) Characterization of the wound-inducible protein ipomoelin from sweet potato. Plant, Cell and Environments 28, 251-259.
27. Chen, Y.-C., Tseng, B.-W., Huang, Y.-L. Liu, Y.-C. and Jeng, S.-T.* (2003) Expression of the ipomoelin gene from sweet potato is regulated by dephosphorylated proteins, calcium ion, and ethylene. Plant, Cell and Environments 26, 1373-1383.
Zong Yan Liu