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Ing-Feng Chang Associate Professor

Ph.D., University of California, Riverside

Specialty: Plant Proteomics, Plant Biochemistry, Molecular Biology


Laboratory: Life Science Building R905

Telephone: 886-2-3366-2534

Current Research Interests


How plants regulate root growth and development How does plant 14-3-3 protein affect physiological responses Plant responses and phosphorylated protein body changes under salt stress

Laboratory Introduction

  • Protein phosphorylation plays an important role in signal transmission and is usually catalyzed by specific kinases. 14-3-3 is scaffold protein. 14-3-3 will bind to phosphorylated protein. The binding protein usually has Mode I (K/R XX S/TXP) or Mode II (K). /R XXX S/TXP) binding motif. These binding proteins include transcription factors, translation factors, membrane receptors and other important proteins. This laboratory uses Arabidopsis as a model organism, uses phosphorylated proteomics and mass spectrometry to identify protein phosphorylation sites, and uses biochemical genetic analysis methods to study the regulation and function of specific protein phosphorylation in salt stress and growth and development. Under salt stress, the activity of some membrane proteins on the membrane of plant cells, in particular, is regulated by phosphorylation to varying degrees. Mass spectrometry can identify these phosphorylation sites regulated by salt and quantify the degree of phosphorylation. In addition, the growth and development of plant roots are affected by internal metabolites or hormones and external environmental factors. Our laboratory has screened mutants with defects in root growth and development to try to find out the mechanism that causes the different phenotypes, and through the exploration of 14-3-3 combination The physiological function of protein, and analyze how 14-3-3 and binding protein affect plant physiology through the combination.


The figure shows that the cytoplasmic calcium ion concentration of Arabidopsis glutamate receptor 3.7 mutant glr3.7-2 is lower under salt stress

Selected Publications

A. Papers

  1. Wang, PH, Lee, CE, Lin, YS, Lee, MH, Chen, PY, Chang, HC, Chang, IF* 2019. The Glutamate Receptor-Like Protein GLR3.7 Interacts With 14-3-3ω and Participates in Salt Stress Response in Arabidopsis thaliana. Frontiers in Plant Science 10:1169

  2. Chang, HC, Tsai, MC, Wu, SS, Chang, IF* 2019. Regulation of ABI5 expression by ABF3 during salt stress responses in Arabidopsis thaliana. Botanical Studies 60:16.

  3. Shih, YJ, Chang, HC, Tsai, MC, Wu, TY, Wu, TC, Ping Kao, P., Wen-Yuan Kao, WY, Chang, IF * 2018. Comparative leaf proteomic profiling of salt-treated natural variants of Imperata cylindrica. Taiwania 63: 171-182.

  4. Singh, SK, Chien, CT, Chang, I,.F.* 2016. The Arabidopsis glutamate receptor-like gene GLR3.6 controls root development by repressing the Kip-related protein gene KRP4. Journal of Experimental Botany 67(6): 1853-69. (SCI)

  5. Wu, TY,Kao, P., Chang, CL, Hsu, PH, Chou, CH, Chang, I,.F.* 2015. Phosphoproteomic profiling of microsomal fractions in leaves of Cogon grass (Imperata cylindrica). Plant OMICS(SCI )

  6. Lee, TC, Xiong, W., Paddock, T., Carrieri, D., Chang, IF, Chiu, HF, Ungerer, J., Juo, SH, Maness, PC, Yu, J. 2015. Engineered xylose utilization enhances bio-products productivity in the cyanobacterium Synechocystis sp. PCC 6803. Metabolic Engineering 30: 179-189. (SCI)

  7. Chen, YT, Shen, CH, Lin, WD, Chu, HA, Huang, BL, Kuo, CI, Yeh, KW, Huang, LC, Chang, IF 2013. Small RNAs of Sequoia sempervirens during rejuvenation and phase change. Plant Biology 15: 27-36. (SCI)

  8. Huang, SJ, Chang, CL, Wang, PH, Tsai, MC, Hsu, PH, Chang, IF* 2013. A type III ACC synthase, ACS7, is involved in root gravitropism in Arabidopsis thaliana. Journal of Experimental Botany 64: 4343 -4360. (SCI)

  9. Chen, YT, Shen, CH, Lin, WD, Chu, HA, Huang, BL, Kuo, CI, Yeh, KW, Huang, LC, Chang, IF 2013. Small RNAs of Sequoia sempervirens during rejuvenation and phase change. Plant Biology 15: 27-36. (SCI)

  10. Chu, HA, Chang, IF, Shen, CH, Chen, YT, Wang, HT, Huang, LC, Yeh, KW 2012. Photosynthetic properties and photosystem stoichiometry of in vitro-grown juvenile, adult, and rejuvenated Sequoia sempervirens (D. Don) Endl. Botanical Studies53: 223-227. (SCI)

  11. Chang, IF*, Hsu, JL, Hsu, PH, Sheng, WA, Lai, SJ, Lee, C., Chen, CW, Hsu, JC, Wang, SY, Wang, LY, Chen, CC 2012. Comparative phosphoproteomic analysis of microsomal fractions of Arabidopsis thaliana and Oryza sativa subjected to high salinity. Plant Science 185-186: 131-142. (SCI)

  12. Curran, A.+, Chang, IF+, Chang, CL Garg, S., Garg, S., Miguel, RM, Barron, YD, Li, Y., Romanowsky, S., Cushman, JC, Gribskov, M., Harmon, AC, Harper, JF* 2011. Calcium-dependent protein kinases from Arabidopsis show substrate specificity differences in an analysis of 103 substrates. Frunt Plant Science 2:36 (+: equal contributions) (SCI)

  13. Chang, IF, Chen, PJ, Shen, CH, Hsieh, TJ, Hsu, YW, Huang, BL, Kuo, CI, Chen, YT, Chu, HA, Yeh, KW, Huang, LC* 2010. Proteomic profiling of proteins associated with the rejuvenation of Sequoia sempervirens (D. Don) Endl. Proteome Science 8: 64. (SCI)

  14. Hsu, JL, Wang, LY, Wang, SY, Lin, CH, Ho, KC, Shi, FK and Chang, IF* 2009. Functional phosphoproteomic profiling of phosphorylation sites in membrane fractions of salt-stressed Arabidopsis thaliana. Proteome Science 7: 42. (SCI)

  15. Chang, IF+*, Curran A.+, Woolsey R., Quilici D., Cushman J., Mittler R., Harmon A., Harper JF* 2009. Proteomic profiling of tandem affinity purified 14-3-3 protein complexes in Arabidopsis thaliana. Proteomics 9: 2697-2985. (+: equal contributions) (SCI)

  16. Hsu, YW, Sihgh, SK, Chiang, MY, Wu, YY, Chang, IF 2009. Strategies to lower greenhouse gas level by rice agriculture. African Journal of Biotechnology 8: 126-132.

  17. Chang, IF 2008. Ecotypic variation of a medicinal plant Imperata cylindrica populations in Taiwan: mass spectrometry-based proteomic evidence. Journal of Medicinal Plants Research 2: 71-76.

  18. Hsiao, HY, Chang, IF 2008. Proteomic profiling of rat brain discharged by ultrasound associated with high frequency electro-magnetic field. Proteomics Research Journal 1: 41-53.

  19. Chang, I. -F. 2006. Mass-spectrometry based proteomic analysis of the epitope-tag affinity purified protein complex in eukaryotes. Proteomics 6: 6158-6166 (Review). (SCI)

  20. Chang, I. -F. and Chou, CH 2006. Ecotypic variation of Imperata cylindrica populations in Taiwan: II. Physiological and biochemical evidences. Botanical Studies 47: 175-184. (SCI)

  21. Rodriguez Milla, MA, Uno, Y., Chang, I. --F., Townsend, J., Maher, EA, Quilici, D. and Cushman JC 2006. Arabidopsis AtCPK11, a calcium-dependent protein kinase, phosphorylates AtDi19, a nuclear zinc finger protein. FEBS Letters 580: 904-911. (SCI)

  22. Rodriguez Milla, MA, Townsend, J., Chang, I. --F. and Cushman, JC 2006. Arabidopsis Di19-Related Genes Encode a Novel Family of Proteins With Two Unusual Cys2/His2 Zinc-Finger Motifs Evolutionary Conserved Involved in ABA- Independent Stress-Signaling Pathways. Plant Molecular Biology 61: 13-30. (SCI)

  23. Chang, I. –F., Hsiao, HY 2005. Induction of RhoGAP and pathological changes characteristic of Alzheimer's disease by UAHFEMF discharge in rat brain. Current Alzheimer Research 2:559-569. (SCI)

  24. Zanetti, ME, Chang, I. -F., Galbraith, DW, Bailey-Serres, J. 2005. Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression. Plant Physiology 138: 624-635. (SCI)

  25. Chang, I. -F.*, Szick-Miranda*, K., Pan, S., Bailey-Serres, J. 2005. (*: equal contributions) Proteomic Characterization of Evolutionarily Conserved and Variable Proteins of Arabidopsis Cytosolic Ribosomes. Plant Physiology 137: 848-862. (SCI)

  26. Yang, CM, Wang, MC, Lu, YF, Chang, I. -F., Chou, CH 2004. Humic substances affect the activity of chlorophyllase. Journal of Chemical Ecology 30: 1057-1065. (SCI)

  27. Yang, CM, Chou, CH, Chang, I. -F. and Lin, SJ 2004. Effects of three allelopathic phenolics on chlorophyll accumulation of rice (Oryza sativa) seedlings: II Stimulation of consumption-orientation. Bot. Bull. Acad. Sin. 45: 119-125. (SCI)

  28. Williams, A.*, Werner-Fraczek. J.*, Chang, I.-F.,* Bailey-Serres, J. (*: equal contributions) 2003. Regulated phosphorylation of 40S ribosomal S6 in root tips of maize. Plant Physiology 132: 2086-2097. (SCI)

  29. Barakat, A., Szick-Miranda, K., Chang, I. -F., Guyot, R., Blanc, G., Cooke, R., Delseny, M., Bailey-Serres, J. 2001. The organization of cytoplasmic ribosomal protein genes in the Arabidopsis genome. Plant Physiology 127: 398-415. (SCI).

B. Book Chapter

Kao, P., Wu, TY, Chang, CL, Chou, CH, Chang, IF 2011. Decreasing of Population Size of Imperata cylindrica Mangrove Ecotype & Sea-Level Rising, Global Warming Impacts-Case Studies on the Economy, Human Health, and on Urban and Natural Environments, Stefano Casalegno (Ed.), ISBN: 978-953-307-785-7, InTech.

Courses Taught

  • General Botany

  • Proteomics

  • Plant Physiology

  • Methods in Plant Molecular Biology

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