Hsu-Liang Hsieh Professor
Ph.D., University of Texas, at Austin, USA
Specialty: Plant Molecular Biology, Phytochrome Signal Transduction, Molecular Genetics
Office Place: Life Science Building R307
Office TEL: 886-2-3366-2526
Laboratory: Life Science Building R919 Laboratory
Plant development is regulated by both extrinsic factors, including light, and intrinsic factors such as genetics and phytohormones. We use Arabidopsis seedlings as a model system to study the integration of light- and multiple hormones-mediated signalings, leading to photomorphogenic development. FIN219/ JAR1, a jasmonate conjugating enzyme that belongs to a GH3 gene family in Arabidopsis, has been shown to participate in phytochrome A (phyA)-mediated far-red (FR) light signaling and can be induced by auxin and jasmonate. FIN219 as an extragenic suppressor of COP1 can interact with different partners to regulate hypocotyl elongation under far-red light condition. Ectopic expression of different domains of FIN219 in wild-type Columbia shows that the N- and C-terminal domains have an opposite effect on photomorphogenic development. Furthermore , our data reveal that the fin219 mutant affects the levels of COP1 and HY5 specifically under FR, but not other light conditions. COP1 and HY5 int eract in the dark, resulting in the degradation of HY5 and leading to a skotomorphogenesis with long hypocotyls and closed cotyledons. In contrast, in the light, COP1 exclusively moves to the cytoplasm and releases HY5 from repression, giving rise to a photomorphogenic development with short hypocotyls, open cotyledons and light responsive gene expression. How the FIN219/JAR1 expression affects the regulation of COP1 and HY5 interaction relationship remains to be elucidated. How jasmonate signaling mediated by FIN219/JAR1 modulates the photomorphogenesis involving COP1 and HY5 regulatory relation is also intriguing . Thus, we plan to use molecular genetics, cell biology and microarray approaches to dissect the functional role of FIN219/JAR1 in the crosstalk between FR light and jasmonate signaling pathways.
A model of FIN219/JAR1 functions in the photomorphogenic development of Arabidopsis seedlings under far-red light.
Huai-Ju Chen, Tsu-Yu Fu, Shao-Li Yang, Hsu-Liang Hsieh* (2018) FIN219/JAR1 and cryptochrome1 antagonize each other to modulate photomorphogenesis under blue light in Arabidopsis. PLOS Genetics March 21, 2018
Swain S, Jiang HW and Hsieh HL* (2017) FAR-RED INSENSITIVE 219/JAR1 contributes to shade avoidance responses of Arabidopsis seedlings by modulating key shade signaling components. Front. Plant Sci. 8:1901.
Chen CY, Ho SS, Kuo TY, Hsieh HL, and Cheng YS* (2017) Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation. PNAS 114 (10): E1815-E1824 .
Leu KC, Hsieh MH, Wang HJ, Hsieh HL, Jauh GY* (2016) Distinct role of Arabidopsis mitochondrial P-type pentatricopeptide repeat protein-modulating editing protein, PPME, in nad1 RNA editing. RNA Biology 13: 593-604.
Chen HJ, Chen CL, and Hsieh HL* (2015) Far-Red light-mediated seedling development in Arabidopsis involves FAR-RED INSENSITIVE 219/ JASMONATE RESISTANT 1-dependent and -independent pathways.PLoS ONE 10 (7): e0132723. doi :10.1371/journal.pone.0132723
Lin LL, Hsu CL, Hu CW, Ko SY, Hsieh HL, Huang* HC and Juan HF* (2015) Integrating phosphoproteomics and bioinformatics to study brassinosteroid-regulated phosphorylation dynamics in Arabidopsis. BMC Genomics 16:533-549. DOI 10.1186/ s12864-015-1753-4
Hsu YW, Wang HJ, Hsieh MH, Hsieh HL and Jauh GY* (2014) Arabidopsis mTERF15 is required for mitochondrial nad2 intron 3 splicing and functional complex I activity. PLOS one 9: e112360. doi:10.1371/journal.pone.0112360.
Hsieh HL and Okamoto* (2014) Molecular interaction of jasmonate and phytochrome A signaling. Journal of Experimental Botany 65: 2847-2857. doi: 10.1093/jxb/eru230
Lin LL, Wu CC, Huang HC*, Chen HJ, Hsieh HL* and Juan HF* (2013) Identification of microRNA 395a in 24-epibrassinolide-regulated root growth of Arabidopsis thaliana using microRNA arrays. Int. J. Mol. Sci. 14: 14270-14286; doi:10.3390/ijms140714270.
Liu XC, Chen CY, Wang KC, Luo M, Tai R, Yuan LY, Zhao ML, Yang SG, Tian G, Cui YH, Hsieh HL and Wu K* (2013) PIF3 associates with the histone deacetylase HDA15 in repression of chlorophyll biosynthesis and photosynthesis in etiolated Arabidopsis seedlings. Plant Cell 25: 1258-1273.
Hsieh WP, Hsieh HL and Wu SH* (2012) Arabidopsis bZIP16 transcription factor integrates light and hormone signaling pathways to regulate early seedling development. Plant Cell 24: 3997-4011.
Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL and Lin TP* (2012) Drought and salt stress tolerance of Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid. Plant Physiology 158: 340-351.
Wang JG and Hsieh HL* (2012) Induction of tomato Jasmonate-Resistant 1-Like 1 gene expression can delay the colonization of Ralstonia solanacearum in transgenic tomato. Botanical Studies 53: 75-84.
Wang JG, Chen CH, Chien CT and Hsieh HL* (2011) FAR-RED INSENSITIVE 219?? modulates CONSTITUTIVE PHOTOMORPHOGENIC 1 activity via physical interaction to regulate hypocotyl elongation in Arabidopsis. Plant Physiology 156: 631-646.
Wu TH, Liao MH, Kuo WY, Huang CH, Hsieh HL and Jinn TL* (2011) Characterization of copper/zinc and manganese superoxide dismutase in green bamboo (Bambusa oldhamii): Cloning, expression and regulation. Plant Physiol Biochem 49: 195 -200.? (1: equal contribution)
Jiang HW, Liu MJ, Chen IC, Huang CH, Chao LY and Hsieh HL* (2010) A Glutathione S-transferase regulated by light and hormones participates in the modulation of Arabidopsis seedling development. Plant Physiology 154: 1646-1658.
Chen IC, Lee SC, Pan SM and Hsieh HL* (2007) GASA4, a GA-stimulated gene, participates in light signaling in Arabidopsis. Plant Science 172: 1062-1071.
Chen IC, Huang IC, Liu MJ, Wang ZG, Chung SS and Hsieh HL* (2007) Glutathione S-Transferase Interacting with FIN219 Is Involved in Phytochrome A-Mediated Signaling in Arabidopsis. Plant Physiology 143: 1189-1202.
Hsieh HL, Okamoto H, Wang M, Ang LH, Matsui M, Goodman M and Deng XW (2000) FIN219, an auxin-regulated gene, defines a genetic link between phytochrome A and the downstream regulator COP1 in light control of Arabidopsis development. Genes & Development 14: 1958-1970.
Hsieh HL, Song CJ, and Roux SJ (2000) Regulation of a recombinant pea nuclear apyrase by calmodulin and casein kinase II. Biochim. Biophys. Acta 1494: 248-255.
Tong CG, Reichler S, Blumental S, Balk J, Hsieh HL and Roux SJ (1997) Light regulation of the abundance of mRNA encoding a nucleolin-like protein localized in the nucleoli of pea nuclei. Plant Physiol 114: 643-652.
Hsieh HL, Tong CG, Thomas C and Roux SJ (1996) Light-regulated mRNA abundance of a gene encoding a calmodulin-regulated, chromatin-associated NTPase in pea. Plant Molecular Biology 30: 135-148.
Hsieh JS, Hsieh HL and Hsing YI (1991) Genetic studies on the Wx gene of sorghum[ Sorghum bicolor(L.) Moench] V. Examination of the activity of Wx protein in developing endosperm. Chinese Agron. J. 1: 35- 45.
Hsieh HL and Hsieh JS (1989) Genetic studies on the Wx gene of sorghum. II. Immunological characterization of Wx geneproduct (Wx protein) in sorghum endosperm. Mem. Coll. Agric. Natl. Taiwan Univ. 29: 23-28.
Plant Growth and Development
Special Topics in Plant Molecular Biology
Study on Plant Functional Genomics